Synthetic tools for studying the chemical biology of InsP8 † †Electronic supplementary information (ESI) available: Data deposition: atomic coordinates and structure factors have been deposited in the Protein Data Bank, www.pdb.org (PDB ID codes 5BYA and 5BYB). See DOI: 10.1039/c5cc05017k
نویسندگان
چکیده
a Wolfson Laboratory of Medicinal Chemistry, Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK. b Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA. c Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
منابع مشابه
First synthetic analogues of diphosphoinositol polyphosphates: interaction with PP-InsP5 kinase† †Electronic supplementary information (ESI) available: Data deposition: the atomic coordinates and structure factors have been deposited in the Protein Data Bank, www.pdb.org (PDB ID code 4GB4). See DOI: 10.1039/c2cc36044f Click here for additional data file.
We synthesised analogues of diphosphoinositol polyphosphates (PP-InsPs) in which the diphosphate is replaced by an α-phosphonoacetic acid (PA) ester. Structural analysis revealed that 5-PA-InsP(5) mimics 5-PP-InsP(5) binding to the kinase domain of PPIP5K2; both molecules were phosphorylated by the enzyme. PA-InsPs are promising candidates for further studies into the biology of PP-InsPs.
متن کاملAzasugar inhibitors as pharmacological chaperones for Krabbe disease† †Electronic supplementary information (ESI) available: Details of IGF, AGF, IGL and DIL syntheses including NMR spectra. Table containing X-ray data collection and refinement statistics. Dose dependence of thermal stabilization of GALC. Controls for DSF assays. IGF- and AGF-mediated stabilization of GALC is buffer and pH dependent. See DOI: 10.1039/c5sc00754b Click here for additional data file. ‡ ‡Data deposition: the atomic coordinates and structure factors have been deposited in the Protein Data Bank, http://www.pdb.org [PDB ID codes 4UFH (IGF), 4UFI (AGF), 4UFJ (IGL), 4UFK (DIL), 4UFL (DGN) and 4UFM (DGJ)].
Content General methods .............................................................................................................................................. S1 Apparatus ......................................................................................................................................................... S2 Abbreviations..................................................
متن کاملA strategy based on nucleotide specificity leads to a subfamily-selective and cell-active inhibitor of N 6-methyladenosine demethylase FTO† †Electronic supplementary information (ESI) available: Experimental details, including full synthesis procedure, T m shift analyses, biochemical and cell-based assay conditions, protein purification methods, crystallisation and structure solution methods. The coordinates and structural factors for FTO in complex with 12, 16 and 21 have been deposited in the RCSB Protein Data Bank as PDB ID 4CXW, 4CXX and 4CXY. See DOI: 10.1039/c4sc02554g Click here for additional data file.
Department of Pharmacy, National Unive 117543, Singapore. E-mail: esther.woon@n 6516 2932 Institute of Molecular and Cell Biology, 61 B School of Biological Sciences, Nanyang Tec 637551, Singapore. E-mail: [email protected] † Electronic supplementary information including full synthesis procedure, T cell-based assay conditions, protein pur structure solution methods. The coordin complex with 12, 1...
متن کاملEngineering proximal vs. distal heme–NO coordination via dinitrosyl dynamics: implications for NO sensor design† †Electronic supplementary information (ESI) available: Structure factors and atomic coordinates have been deposited in the RCSB Protein Data Bank with accession codes; 5JT4, 5JLI, 5JP7, 5JRA, 5JVE, 5JUA, 5JSL, 5JS5. See DOI: 10.1039/c6sc04190f Click here for additional data file.
School of Biological Sciences, University of CO4 3SQ, UK. E-mail: [email protected] Department of Chemistry and Biochemistry Oregon 97850, USA. E-mail: candrew@eou. Faculty of Science and Education Science, Iraq Medical Research Center, Hawler Medical Swiss Light Source, Paul Scherrer Institute, Manchester Institute of Biotechnology, 131 School of Chemistry, Bangor University, Ba Molecular Bio...
متن کاملH2O2-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin† †Electronic supplementary information (ESI) available: Information on materials, instrumentation, experimental details and additional data on preparation of proteins, crystal structure analysis, resonance Raman and FTIR spectroscopy, reaction of reduced I119H with O2, consumption of H2O2, and oxidation reactions of guaiacol and 1,4-cyclohexadiene. The atomic coordinates and structure factors (PDB code 3WHN) have been deposited into the Protein Data Bank, http://www.rcsb.org/. See DOI: 10.1039/c3cc48108e Click here for additional data file.
The O2-binding carboxylate-bridged diiron site in DcrH-Hr was engineered in an effort to perform the H2O2-dependent oxidation of external substrates. A His residue was introduced near the diiron site in place of a conserved residue, Ile119. The I119H variant promotes the oxidation of guaiacol and 1,4-cyclohexadiene upon addition of H2O2.
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